The 5 Hardest Level 2 Anatomy and Physiology Exam Questions

The 5 Hardest Level 2 Anatomy and Physiology Exam Questions (and Answers)

This blog contains the 5 hardest Level 2 Anatomy and Physiology Exam Questions, with answers and explanations. Try each of these mock questions to check you are truly ready for the hardest questions in your anatomy exam.

You’ll discover:

  • How to use the 5 hardest Level 2 Anatomy and Physiology Exam Questions to improve your revision
  • What to expect in your exam
  • Five Mock Questions are taken from recent Level 2 anatomy exams
  • Answers and explanations for each mock question
  • Useful links to expand your knowledge
  • How to simplify and structure your revision to pass with confidence

How to use these Mock Questions

Look at the 5 questions below and jot down your answer on a scrap paper or as a note in your phone.

Then scroll down to reveal the answers and also get an explanation and further resources to help you truly understand the CORRECT answers.

What Can I expect on Exam Day?

There is usually 50 multiple choice questions in your Level 2 Anatomy and Physiology exam [this varies per training provider] and a 115-minute time limit.

In order to pass, you must score a minimum of 35 out of 50 correct answers (or 70%).

This can cause anxiety as these 50 questions are spread across 8 modules of information and can include anything from muscle structure and joint actions to blood circulation and energy systems.

Teaching the Level 2 fitness instructor qualification for over 10 years, we have seen a lot of exam papers and voted on what we believe to be the 5 hardest Active IQ level 2 anatomy and physiology exam questions.

Here I’m going to share them with you, and I explain the answer too 🙂

The 5 Hardest Level 2 Anatomy and Physiology Exam Questions:

1) Which of the following describes the correct sequence of blood flow?

A. Pulmonary vein, right ventricle, right atrium, pulmonary artery
B. Vena cavae, left atrium, left ventricle, aorta
C. Pulmonary vein, left atrium, left ventricle, aorta
D. Aorta, left atrium, left ventricle, pulmonary artery

2) Which of the following describes the role of a synergist muscle?

A. A muscle which relaxes to allow movement
B. The main muscle involved in the action
C. The muscle which assists the main muscle in bringing about the action
D. A muscle which contracts statically to fix another body part

3) Bundles of muscle fibres are wrapped within the?

A. Myofibril
B. Periosteum
C. Myofilament
D. Perimysium

4) Which of the following types of movement is possible at the knee joint?

A. Lateral flexion
B. Extension
C. Adduction
D. Abduction

5) Which of the following muscles is involuntary?

A. Cardiac Muscle
B. Biceps Brachii Muscle
C. Hamstring Muscle
D. Spinal Cord


Q1: Answer = C

Explanation: Pulmonary vein, left atrium, left ventricle, aorta

This video explains the sequence of blood flow in just 2 minutes..

The Entire Heart and Circulatory System in 2 Minutes

Q2: Answer = C

Explanation: The muscle which assists the main muscle in bringing about the action.

The synergist in a movement is the muscle(s) that stabilises a joint around which movement is occurring, which in turn helps the agonist function effectively.

Synergist muscles also help to create the movement.

Example: In the bicep curl the synergist muscles are the brachioradialis and brachialis which assist the biceps to create the movement and stabilise the elbow joint.

Q3: Answer = D

Explanation: Perimysium

Each skeletal muscle fibre is a single cylindrical muscle cell. An individual skeletal muscle may be made up of hundreds, or even thousands, of muscle fibres bundled together and wrapped in a connective tissue covering.

Each muscle is surrounded by a connective tissue sheath called the epimysium. Fascia, connective tissue outside the epimysium, surrounds and separates the muscles. Portions of the epimysium project inward to divide the muscle into compartments. Each compartment contains a bundle of muscle fibers. 

Each bundle of muscle fibres is called a fascicle and is surrounded by a layer of connective tissue called the perimysium. 

Within the fasciculus, each individual muscle cell, called a muscle fiber, is surrounded by connective tissue called the endomysium.

Q4: Answer = B

Explanation:  Extension

The knee joint is a hinge type synovial joint, which mainly allows for flexion and extension (and a small degree of medial and lateral rotation).

Movements: There are four main movements that the knee joint permits. You will need to know about just two of these for your Level 2 Anatomy and Physiology Exam Questions

Extension: Produced by the quadriceps.

Flexion: Produced predominantly by the hamstrings (assisted by the gracilis, sartorius and popliteus).

xplanation: A. Cardiac Muscle

Cardiac muscle is an involuntary muscle, found only in the heart. The cardiac muscle is striated, and meaning all muscle fibres contract together, generating enough force to push blood throughout the body.

Q5: Answer = A

There are two other types of muscle:

1) Skeletal muscle is made up of voluntary muscles, usually attached to the skeleton. Skeletal muscles move the body for example the Biceps Brachii is a skeletal muscle.

These muscle fibres are also striated, meaning they can create a large force.

2) Smooth muscle is composed of involuntary muscles found within the walls of organs and structures such as the esophagus, stomach, intestines, and blood vessels. 

These muscles push materials like food or blood through organs. Unlike skeletal muscle, smooth muscle can never be under your control.

Download more Level 2 Anatomy and Physiology Exam Questions

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Hayley “Level 2 Anatomy and Physiology Exam Questions” Bergman

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